Use of biological methods and addition of organic substances in anaerobic fermentation of biomass to increase methane production and reduce carbon dioxide level – CIAS Journal – CIAS Journal
Research Article
Volume 3 | Issue 3 (July - Sept) |Article ID CIAS0098 | https://doi.org/10.65791/cias.98

Use of biological methods and addition of organic substances in anaerobic fermentation of biomass to increase methane production and reduce carbon dioxide level


Osuji Malachy Ikeokwu iD1, Amai Innocent Ugochukwu1, Nwabufoh Onyeka2

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Academic Editor: Dr. Anantha Rama A
Recieved
10 Jan 2026
Revised
13 May 2026
Accepted
24 Jun 2026
Published
01 Jul 2026

Abstract

This research work was done to ascertain the percentage composition of methane produced and the level of carbon dioxide when some organic substances were added to the fermentation tank during biogas production. The following: bovine blood, magnesium sulphate, charcoal water, and water of pH 8 were used as additives. After 21 days of batch culture fermentation, the individual gases were analyzed using gas chromatography. For gas from slurry amended with water of pH 8, the total gas concentration was found to be 18.38. In this gas concentration, methane, which is the primary gas sought in biogas production, had a concentration of 10.15. This was followed by carbon dioxide, which had a concentration of 2.39, corresponding to a percentage composition of 13.01%. For the slurry with MgSO₄, it was found that the total gas concentration in the feedstock amended with magnesium sulphate was 23.19. Carbon dioxide, on the other hand, had a concentration of 3.06 and a percentage composition of 13.20%. The analysis of the gas produced from the feedstock treated with charcoal water revealed a total gas concentration of 220.42. In this gas concentration, methane, the primary gas sought after in biogas production, had a concentration of 171.76. This concentration corresponds to a percentage composition of 77.92%. Carbon dioxide and carbon monoxide exhibited the lowest concentrations and percentage compositions. The four gases analyzed showed that the levels of methane and carbon dioxide have a significant effect on their levels.


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